rabbit anti cc3 Search Results


rabbit monoclonal anti-cc3 ( :500  (Becton Dickinson)
90
Becton Dickinson rabbit monoclonal anti-cc3 ( :500
(A) Confocal image of P3 retinal flat mount (Arrowhead and magnified boxes illustrate interactions) in NFL/GCL. CD11c-GFP+ microglia (green); cleaved caspase 3, <t>CC3</t> (pink); and RBPMS (blue). Scale bar, 50μm. (B) Percent CD11c-GFP+ microglia contacting apoptotic RGCs (RBPMS+CC3+) at P3 out of total CD11c-GFP+ microglia within GCL. (n=5 animals). (C) Confocal images of retinal flat mounts of P3 CD11c-GFP animals in GCL showing a range of microglial GFP expression from none to high. Scale bar, 10μm. Dashed line demarcates distinction between CD11c-GFP negative and positive. (D) Cell area immunostained for CD68 ( Top ) and C1q ( Bottom ) in CD11c-GFP- and CD11c-GFP+ microglia (n=4 animals, 352 GFP-cells and 422 GFP+ cells). Mann-Whitney test Top **P =. 0011 and Bottom ****P <. 0001. (E) Scatter plot of CD68 area/cell compared to CD11c-GFP area/cell at P3 in GCL. Pearson r= 0.4145 **** P < .0001. (F) Flow cytometry analysis showing the percent CD11c Hi of total CD45+CD11b+ or CD45+CX3CR1-GFP+ microglia from retinas across all genotypes. CX3CR1-GFP/+ (n=10), CX3CR1-KO (n=6), CR3 KO (n=6), MerTK KO (n=9), Axl KO (n=8), and MerTK/Axl dKO (n=6), ≥ 2 litters collected for each genotype, ± SEM. One-way ANOVA F(5, 39) = 34.64 P <.0001 and Tukey’s multiple comparisons. **** P <.0001, *** P =.0003, ** P =.0056, * P =.02, NS, not significant. (G) Max projected confocal image of P5 retinal flat mount from WT and AXL KO, Spp1+ (green) and total microglia (IBA1+; red). Scale bar, 100μm. (H) Density of Spp1+IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test * P = .0139. (I) Percent Spp1+IBA1+ of total IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test ***P <.001
Rabbit Monoclonal Anti Cc3 ( :500, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+cc3/rabbit+monoclonal+anti+cc3+++++500/bio_rxiv__2024__06__24__600082-138-30-35
Average 90 stars, based on 1 article reviews
rabbit monoclonal anti-cc3 ( :500 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rabbit anti-cc3 antibody  (Merck KGaA)
90
Merck KGaA rabbit anti-cc3 antibody
(A) Confocal image of P3 retinal flat mount (Arrowhead and magnified boxes illustrate interactions) in NFL/GCL. CD11c-GFP+ microglia (green); cleaved caspase 3, <t>CC3</t> (pink); and RBPMS (blue). Scale bar, 50μm. (B) Percent CD11c-GFP+ microglia contacting apoptotic RGCs (RBPMS+CC3+) at P3 out of total CD11c-GFP+ microglia within GCL. (n=5 animals). (C) Confocal images of retinal flat mounts of P3 CD11c-GFP animals in GCL showing a range of microglial GFP expression from none to high. Scale bar, 10μm. Dashed line demarcates distinction between CD11c-GFP negative and positive. (D) Cell area immunostained for CD68 ( Top ) and C1q ( Bottom ) in CD11c-GFP- and CD11c-GFP+ microglia (n=4 animals, 352 GFP-cells and 422 GFP+ cells). Mann-Whitney test Top **P =. 0011 and Bottom ****P <. 0001. (E) Scatter plot of CD68 area/cell compared to CD11c-GFP area/cell at P3 in GCL. Pearson r= 0.4145 **** P < .0001. (F) Flow cytometry analysis showing the percent CD11c Hi of total CD45+CD11b+ or CD45+CX3CR1-GFP+ microglia from retinas across all genotypes. CX3CR1-GFP/+ (n=10), CX3CR1-KO (n=6), CR3 KO (n=6), MerTK KO (n=9), Axl KO (n=8), and MerTK/Axl dKO (n=6), ≥ 2 litters collected for each genotype, ± SEM. One-way ANOVA F(5, 39) = 34.64 P <.0001 and Tukey’s multiple comparisons. **** P <.0001, *** P =.0003, ** P =.0056, * P =.02, NS, not significant. (G) Max projected confocal image of P5 retinal flat mount from WT and AXL KO, Spp1+ (green) and total microglia (IBA1+; red). Scale bar, 100μm. (H) Density of Spp1+IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test * P = .0139. (I) Percent Spp1+IBA1+ of total IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test ***P <.001
Rabbit Anti Cc3 Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+cc3/rabbit+anti+cc3+antibody/pm33545716-87-12-15
Average 90 stars, based on 1 article reviews
rabbit anti-cc3 antibody - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


(A) Confocal image of P3 retinal flat mount (Arrowhead and magnified boxes illustrate interactions) in NFL/GCL. CD11c-GFP+ microglia (green); cleaved caspase 3, CC3 (pink); and RBPMS (blue). Scale bar, 50μm. (B) Percent CD11c-GFP+ microglia contacting apoptotic RGCs (RBPMS+CC3+) at P3 out of total CD11c-GFP+ microglia within GCL. (n=5 animals). (C) Confocal images of retinal flat mounts of P3 CD11c-GFP animals in GCL showing a range of microglial GFP expression from none to high. Scale bar, 10μm. Dashed line demarcates distinction between CD11c-GFP negative and positive. (D) Cell area immunostained for CD68 ( Top ) and C1q ( Bottom ) in CD11c-GFP- and CD11c-GFP+ microglia (n=4 animals, 352 GFP-cells and 422 GFP+ cells). Mann-Whitney test Top **P =. 0011 and Bottom ****P <. 0001. (E) Scatter plot of CD68 area/cell compared to CD11c-GFP area/cell at P3 in GCL. Pearson r= 0.4145 **** P < .0001. (F) Flow cytometry analysis showing the percent CD11c Hi of total CD45+CD11b+ or CD45+CX3CR1-GFP+ microglia from retinas across all genotypes. CX3CR1-GFP/+ (n=10), CX3CR1-KO (n=6), CR3 KO (n=6), MerTK KO (n=9), Axl KO (n=8), and MerTK/Axl dKO (n=6), ≥ 2 litters collected for each genotype, ± SEM. One-way ANOVA F(5, 39) = 34.64 P <.0001 and Tukey’s multiple comparisons. **** P <.0001, *** P =.0003, ** P =.0056, * P =.02, NS, not significant. (G) Max projected confocal image of P5 retinal flat mount from WT and AXL KO, Spp1+ (green) and total microglia (IBA1+; red). Scale bar, 100μm. (H) Density of Spp1+IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test * P = .0139. (I) Percent Spp1+IBA1+ of total IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test ***P <.001

Journal: bioRxiv

Article Title: CD11c-expressing microglia are transient, driven by interactions with apoptotic cells

doi: 10.1101/2024.06.24.600082

Figure Lengend Snippet: (A) Confocal image of P3 retinal flat mount (Arrowhead and magnified boxes illustrate interactions) in NFL/GCL. CD11c-GFP+ microglia (green); cleaved caspase 3, CC3 (pink); and RBPMS (blue). Scale bar, 50μm. (B) Percent CD11c-GFP+ microglia contacting apoptotic RGCs (RBPMS+CC3+) at P3 out of total CD11c-GFP+ microglia within GCL. (n=5 animals). (C) Confocal images of retinal flat mounts of P3 CD11c-GFP animals in GCL showing a range of microglial GFP expression from none to high. Scale bar, 10μm. Dashed line demarcates distinction between CD11c-GFP negative and positive. (D) Cell area immunostained for CD68 ( Top ) and C1q ( Bottom ) in CD11c-GFP- and CD11c-GFP+ microglia (n=4 animals, 352 GFP-cells and 422 GFP+ cells). Mann-Whitney test Top **P =. 0011 and Bottom ****P <. 0001. (E) Scatter plot of CD68 area/cell compared to CD11c-GFP area/cell at P3 in GCL. Pearson r= 0.4145 **** P < .0001. (F) Flow cytometry analysis showing the percent CD11c Hi of total CD45+CD11b+ or CD45+CX3CR1-GFP+ microglia from retinas across all genotypes. CX3CR1-GFP/+ (n=10), CX3CR1-KO (n=6), CR3 KO (n=6), MerTK KO (n=9), Axl KO (n=8), and MerTK/Axl dKO (n=6), ≥ 2 litters collected for each genotype, ± SEM. One-way ANOVA F(5, 39) = 34.64 P <.0001 and Tukey’s multiple comparisons. **** P <.0001, *** P =.0003, ** P =.0056, * P =.02, NS, not significant. (G) Max projected confocal image of P5 retinal flat mount from WT and AXL KO, Spp1+ (green) and total microglia (IBA1+; red). Scale bar, 100μm. (H) Density of Spp1+IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test * P = .0139. (I) Percent Spp1+IBA1+ of total IBA1+ microglia in AXL WT and KO (n=5, n=4 animals) ± SEM. Unpaired t test ***P <.001

Article Snippet: Antibodies used include Goat polyclonal anti-GFP ( :2000, Abcam ab5450, RRID: AB_304897), Rabbit monoclonal anti-C1q ( :1500, Abcam an182451, RRID: AB_2732849), Rabbit polyclonal anti-IBA1 ( :1000, Wako 019-19741, RRID: AB_839504), Rabbit monoclonal anti-CC3 ( :500, BD Biosciences 559565, RRID: AB_397274), Guinea pig polyclonal anti-RBPMS ( :750, Millipore Sigma ABN1376, RRID: AB_2687403), Rat monoclonal anti-CD68 ( :250, Bio-Rad MCA1957, RRID: AB_322219), and Goat polyclonal anti-Osteopontin ( :100, Thermo Fischer PA5-34579, RRID: AB_2551931).

Techniques: Expressing, MANN-WHITNEY, Flow Cytometry

(A) Depiction of two depletion strategies, Diphtheria toxin (DT) targeted ablation of CD11c-DTR/GFP+ microglia (Top) and PLX3397 (PLX)-mediated depletion to target more homeostatic microglia (Bottom). (B) Confocal images of CD11c-DTR/GFP+ microglia (green) in retinal flat mounts at P5 of Vehicle and DT-treated CD11c-DTR/GFP mice. Scale bar, 50μm. (C) Density of CD11c-DTR/GFP+ microglia in vehicle and DT-treated CD11c-DTR/GFP (n=6, n=7 animals) ± SEM. Unpaired t test **** P <.0001. (D) Confocal images of C1q+ microglia (white) in immunostained retinal flat mounts at P5 of vehicle and DT-treated CD11c-DTR/GFP mice. Scale bar, 50μm. (E) Densities of C1q+ cells in retinas of vehicle and DT-treated CD11c-DTR/GFP mice (n=6, n=7 animals) ± SEM. Mann Whitney test *P = .0140. (F) Max projected confocal images of C1q+ microglia in retinal flat mounts at P5 of vehicle and PLX-treated Cx3CR1/GFP+ mice. C1q (white). Scale bar, 50μm. (G) Density of C1q+ cells in retinas of naïve and PLX-treated CX3CR1-GFP (n=6, n=8) ± SEM. Unpaired t test *P = .0239. (H) Max projected confocal images of apoptotic RGCs in retinal flat mounts at P5 of all conditions/genotypes. CC3 (magenta); RBPMS (green). Scale bar, 50μm. (I) Density of CC3+RBPMS+ cells in retinas from vehicle and DT-treated CD11c-DTR/GFP mice and DT-treated wildtype mice (WT) (n=8, n=10, n=10 animals respectively) ± SEM. Ordinary one-way ANOVA **P = .0018 and Tukey’s multiple comparisons test: Veh vs DT ** P =.006, WT+DT vs DT **P = .0043, NS , not significant. (J) Density of CC3+RBPMS+ cells in naïve and PLX-treated CX3CR1-GFP/+ (n=8, n=8 animals) ± SEM. Unpaired t test **** P <.0001 (K) Scatter plot of dying RGC density (CC3+RBPMS+/mm 2 ) compared to microglial density (C1q+/mm 2 ) of same animal (n=4 CD11c + vehicle, n=5 CD11c + DT, n=10 PLX animals). Pearson r= -0.6350 ** P =.0035.

Journal: bioRxiv

Article Title: CD11c-expressing microglia are transient, driven by interactions with apoptotic cells

doi: 10.1101/2024.06.24.600082

Figure Lengend Snippet: (A) Depiction of two depletion strategies, Diphtheria toxin (DT) targeted ablation of CD11c-DTR/GFP+ microglia (Top) and PLX3397 (PLX)-mediated depletion to target more homeostatic microglia (Bottom). (B) Confocal images of CD11c-DTR/GFP+ microglia (green) in retinal flat mounts at P5 of Vehicle and DT-treated CD11c-DTR/GFP mice. Scale bar, 50μm. (C) Density of CD11c-DTR/GFP+ microglia in vehicle and DT-treated CD11c-DTR/GFP (n=6, n=7 animals) ± SEM. Unpaired t test **** P <.0001. (D) Confocal images of C1q+ microglia (white) in immunostained retinal flat mounts at P5 of vehicle and DT-treated CD11c-DTR/GFP mice. Scale bar, 50μm. (E) Densities of C1q+ cells in retinas of vehicle and DT-treated CD11c-DTR/GFP mice (n=6, n=7 animals) ± SEM. Mann Whitney test *P = .0140. (F) Max projected confocal images of C1q+ microglia in retinal flat mounts at P5 of vehicle and PLX-treated Cx3CR1/GFP+ mice. C1q (white). Scale bar, 50μm. (G) Density of C1q+ cells in retinas of naïve and PLX-treated CX3CR1-GFP (n=6, n=8) ± SEM. Unpaired t test *P = .0239. (H) Max projected confocal images of apoptotic RGCs in retinal flat mounts at P5 of all conditions/genotypes. CC3 (magenta); RBPMS (green). Scale bar, 50μm. (I) Density of CC3+RBPMS+ cells in retinas from vehicle and DT-treated CD11c-DTR/GFP mice and DT-treated wildtype mice (WT) (n=8, n=10, n=10 animals respectively) ± SEM. Ordinary one-way ANOVA **P = .0018 and Tukey’s multiple comparisons test: Veh vs DT ** P =.006, WT+DT vs DT **P = .0043, NS , not significant. (J) Density of CC3+RBPMS+ cells in naïve and PLX-treated CX3CR1-GFP/+ (n=8, n=8 animals) ± SEM. Unpaired t test **** P <.0001 (K) Scatter plot of dying RGC density (CC3+RBPMS+/mm 2 ) compared to microglial density (C1q+/mm 2 ) of same animal (n=4 CD11c + vehicle, n=5 CD11c + DT, n=10 PLX animals). Pearson r= -0.6350 ** P =.0035.

Article Snippet: Antibodies used include Goat polyclonal anti-GFP ( :2000, Abcam ab5450, RRID: AB_304897), Rabbit monoclonal anti-C1q ( :1500, Abcam an182451, RRID: AB_2732849), Rabbit polyclonal anti-IBA1 ( :1000, Wako 019-19741, RRID: AB_839504), Rabbit monoclonal anti-CC3 ( :500, BD Biosciences 559565, RRID: AB_397274), Guinea pig polyclonal anti-RBPMS ( :750, Millipore Sigma ABN1376, RRID: AB_2687403), Rat monoclonal anti-CD68 ( :250, Bio-Rad MCA1957, RRID: AB_322219), and Goat polyclonal anti-Osteopontin ( :100, Thermo Fischer PA5-34579, RRID: AB_2551931).

Techniques: MANN-WHITNEY